# Problem with Influenza H5N1 tanglegram

**URL:** https://nextstrain.discourse.group/t/problem-with-influenza-h5n1-tanglegram/1615
**Category:** Help and Getting Started
**Created:** [April 17, 2024, 9:33pm UTC](https://nextstrain.discourse.group/t/problem-with-influenza-h5n1-tanglegram/1615 "2024-04-17T21:33:11Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![leocaserta](https://avatars.discourse-cdn.com/v4/letter/l/3d9bf3/32.png) [@leocaserta](https://nextstrain.discourse.group/u/leocaserta)
#### Post date: [April 17, 2024, 9:33pm UTC](https://nextstrain.discourse.group/t/problem-with-influenza-h5n1-tanglegram/1615/1 "2024-04-17T21:33:11Z")

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Hello,  
I dragged and dropped .jason files for HA and NA into auspice, and was expecting to see a tanglegram connecting all the nodes in both trees, but only a few of them have lines connecting (see image below). The sample descriptions are exactly the same between both trees, so I don’t understand why the tanglegram is not working.

 ![image](https://canada1.discourse-cdn.com/flex031/uploads/nextstrain/original/1X/5cac16ffa41566e7552661c2dcea4319e1630c6c.png)

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### Author: ![joverlee](https://avatars.discourse-cdn.com/v4/letter/j/e495f1/32.png) [@joverlee](https://nextstrain.discourse.group/u/joverlee)
#### Post date: [April 18, 2024, 5:17pm UTC](https://nextstrain.discourse.group/t/problem-with-influenza-h5n1-tanglegram/1615/2 "2024-04-18T17:17:18Z")

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Hi @leocaserta,

The tangletree only connects samples that have the exact same name. For example, if you look at the tangletree for the Nextstrain avian flu builds at [https://nextstrain.org/flu/avian/h5n1/ha:flu/avian/h5n1/na](https://nextstrain.org/flu/avian/h5n1/ha:flu/avian/h5n1/na), you can see many samples are connected, but not all of them.

If you are able to share your JSONs, I can help you take a look at why your samples are not connecting as expected.

Best,  
Jover

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### Author: ![leocaserta](https://avatars.discourse-cdn.com/v4/letter/l/3d9bf3/32.png) [@leocaserta](https://nextstrain.discourse.group/u/leocaserta)
#### Post date: [April 19, 2024, 2:10am UTC](https://nextstrain.discourse.group/t/problem-with-influenza-h5n1-tanglegram/1615/3 "2024-04-19T02:10:10Z")

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Hi @joverlee , thank you for your help.  
I think I found out the problem. Some strain names were lower case in a tree while they were upper case in the other.

Leonardo
